What is the recommended method for preserving a split-thickness skin graft for autotransplantation?

• A. Place in Balanced Salt Solution
• B. Refrigerate in Normal Saline
• C. Sterilize with Ethylene Oxide
• D. Steam Sterilization

Answer: (B)

To keep a split-thickness skin graft viable for autotransplantation, the graft must be kept moist and cool. Refrigerating the graft in sterile normal saline provides an isotonic, non-toxic environment while slowing cellular metabolism, which helps preserve cell viability for transfer. This approach maintains hydration without introducing harmful agents or heat. In contrast, sterilization methods or high heat would destroy tissue, and drying or desiccation would rapidly injure the graft. Wrapping the graft in sterile saline–moistened gauze and placing it on ice is a practical way to implement this preservation method.